APTT ELLAGIC ACID Valuation of activated Partial Thromboplastin Time (APTT) in plasma use manual and automatic procedure TEST SUMMARY
The contact system is activated by the ellagic acid.
• As with any diagnostic procedure, if the results are
The cephalic extracted from bovine brain take effect as
inconsistent with the clinical presentation, the
substitute for PF3. The clot formation begins with the
physician should evaluate data obtained using this
addition of calcium chloride. APTT determination is
test in light of other clinical information.
sensitive to deficiencies in activities of II, V, VIII, IX, X,
XI and XII factors due to hereditary disorders, liver
disease, deficiencies of K vitamin and may drug.
CALIBRATION / QUALITY CONTROL
Activated Partial Thromboplastin Time is also sensitive
Normal and Pathological control are recommended for
A pool of normal human plasma can be used as
calibrator plasmas as an alternative to commercial
Collected, into the plastic or siliconized glass tube, 9
parts of freshly draw venous blood and 1 part of
In this case it is important to exclude from the pool of
plasmas subjects illness with estrogen-progesterone
The plasma was separated after centrifugation of the
Stability: 4 hours at 15-25°C or 24 hours at -20°C if
TEST PERFORMANCE
immediately freeze. Do not use EDTA or heparin
Precision REAGENTS INTRA - ASSAY (sec.) REAGENTS PREPARATION AND STORAGE INTER - ASSAY (sec.)
Reagents are ready to use. Unopened reagent and control are stable until the expiration date shown on
Shake Activator vigorously before use. Opened reagent are stable 5 days at 2-8°C.
Do not freeze. Interferences MATERIAL REQUIRED BUT NOT SUPPLIED
Icteric and turbid plasmas can influence the APTT.
Test tube for analysis, chronometer, Calibrator (for
Were detected interference from drugs. Further tests
result obtained in ‘ratio’), Normal Control, Pathological
will be needed to determine the cause of unexpected
Control L, Pathological Control H, Diluent.
PRECAUTION WASTE DISPOSAL
Reagent may contain not reactive and conservative
Product is intended for professional laboratories.
components. It is opportune to avoid contacts with the
Waste products must be handled as per relevant
Perform the test according to the general “Good
Laboratory Practice” (GLP) guidelines.
PACKAGING CODE CG00200 PROCEDURE Manual Application
Shake Activator vial (A) vigorously before use.
CODE CG00210
Put in test-tube analysis 100 µl of sample and 100 µl
REFERENCES
Add 100 µl of Starter (B) prewarm. Value the time clot formation with chronometer.
Bell, W.N. Alton, H.G.: Nature 174.880, 1954.
Hoffmann, I.J.M.L., Neulendijk, P.N.: Thrombos.
EXPECTED VALUES
Haemostas, 39.640, 1978. Biggs, R., Rizza, C. ed.: Human Blood Coagulation,
Every laboratory should be establish own reference
Haemostasis and Thrombosis, III ed. Blackwell
intervals in relation to own population.
Scientific Publications, Oxford, England, 1984.
Shapiro, G.A., Huntzinger, S.W., Wilson, J.E.: Am. J.
MANUFACTURER CLINICAL SIGNIFICANCE
Activity values bigger than those of reference were
reported with deficiency of VIII, IX, X, XI or XII Factor,
kininogen of high molecular weight of prekallikrein,
fibrinogen, of V or II factors (or their inhibitors). High
values were also observed in the DIC, in the presence
of specific inhibitors (LAC), liver diseases and
Small values of APTT are reported for all those states
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APTT ELLAGIC ACID